La Jolla scientists eavesdrop on communication between fat and brain

Ardem Patapoutian
Nobel Prize winner Ardem Patapoutian of Scripps Research in La Jolla was one of the senior authors of a new study on how sensory neurons carry messages from fat to the brain.
(K.C. Alfred / The San Diego Union-Tribune)

Sensory neurons newly discovered by Scripps Research send messages from fat tissue to the brain and could offer clues about obesity and other conditions.


What did the fat say to the brain?

For years, it was assumed that hormones passively floating through the blood were the way a person’s fat could send information related to stress and metabolism to the brain. Now, scientists at Scripps Research in La Jolla report in Nature that newly identified sensory neurons carry a stream of messages from fat — called adipose tissue — to the brain.

“The discovery of these neurons suggests for the first time that your brain is actively surveying your fat, rather than just passively receiving messages about it,” said study co-senior author Li Ye, the Abide-Vividion chair in chemistry and chemical biology and an associate professor of neuroscience at Scripps Research. “The implications of this finding are profound.”

“This is yet another example of how important sensory neurons are to health and disease in the human body,” said co-senior author and professor Ardem Patapoutian, a Nobel laureate and Howard Hughes Medical Institute investigator. Patapoutian’s 2021 Nobel Prize in medicine or physiology was for discovering skin receptors that enable people to sense heat, cold, pain, touch and sound.

In mammals, adipose tissue stores energy in the form of fat cells and releases those stores when the body needs energy. It also controls a host of hormones and signaling molecules related to hunger and metabolism. In conditions including diabetes, fatty liver disease, atherosclerosis and obesity, that energy storage and signaling often go awry.

Researchers have long known that nerves extend into adipose tissue but suspected they weren’t sensory neurons that carry data to the brain. Instead, most hypothesized that the nerves in fat belonged mostly to the sympathetic nervous system — the network responsible for our fight-or-flight response, which switches on fat-burning pathways during times of stress and physical activity. Attempts to clarify the types and functions of these neurons have been difficult; methods used to study neurons closer to the surface of the body or in the brain don’t work well deep in adipose tissue, where nerves are hard to see or stimulate.

Ye and colleagues developed two new methods that enable them to overcome those challenges. First, an imaging approach called HYBRiD turned mouse tissues transparent and allowed the team to better track the paths of neurons as they snaked into adipose tissue. The researchers discovered that nearly half of the neurons didn’t connect to the sympathetic nervous system but instead to dorsal root ganglia — an area of the brain where all sensory neurons originate.

To better probe the role of these neurons in adipose tissue, the group turned to a second new technique that it named ROOT, for “retrograde vector optimized for organ tracing.” ROOT let the researchers selectively destroy small subsets of sensory neurons in the adipose tissue using a targeted virus and then observe what happened.

“This research was really made possible by the way these new methods came together,” said Yu Wang, a graduate student in both the Ye and Patapoutian labs and first author of the new paper. “When we first started this project, there weren’t existing tools to answer these questions.”

The experiments revealed that when the brain doesn’t receive sensory messages from adipose tissue, programs triggered by the sympathetic nervous system — related to the conversion of white fat to brown fat — become overly active in fat cells, resulting in a larger-than-normal fat pad with especially high levels of brown fat, which breaks down other fat and sugar molecules to produce heat. Indeed, the animals with blocked sensory neurons — and high levels of sympathetic signaling — had increased body temperatures.

The findings suggest that the sensory neurons and sympathetic neurons might have two opposing functions, with sympathetic neurons needed to turn on fat burning and the production of brown fat, and sensory neurons required to turn those programs down.

“This tells us that there’s not just a one-size-fits-all instruction that the brain sends adipose tissue,” Ye said. “It’s more nuanced than that; these two types of neurons are acting like a gas pedal and a brake for burning fat.”

The team doesn’t yet know exactly what messages the sensory neurons convey to the brain from adipose tissue, only that the connections and communications are key for keeping fat healthy. The scientists are planning more research into what the neurons are sensing and whether other similar cells exist in additional internal organs.

— La Jolla Light staff contributed to this report.